Comparison of the cytotoxic effects local anesthetics equine

[Guillermo Rodriguez]

Comparison of the cytotoxic effects of bupivacaine,
lidocaine, and mepivacaine in equine articular
chondrocytes
By: Jinuk Park, Bibek C Sutradhar, Gyeongmi Hong, Seok H Choi & Gonhyung Kim

Veterinary Anaesthesia and Analgesia, 2011; 38: Pages 127–133.

Abstract



Objective: To compare the chondrotoxicity of bupivacaine,
lidocaine, and mepivacaine in equine
articular chondrocytes in vitro.
Study design Prospective, experimental study.
Study material Equine articular chondrocytes.
Methods: Primary cultured equine chondrocytes
were exposed to 0.5% bupivacaine, 2% lidocaine,
or 2% mepivacaine for 30 or 60 minutes. After
treatment, cell viability was evaluated by trypan
blue exclusion and the 3-(4,5-dimethylthiazol-2-yl)-
2,5-diphenyltetrazolium bromide (MTT) colorimetric
assay in a dose dependent manner. Apoptosis
and necrosis of chondrocytes were analyzed with
the double staining of Hoechst 33258 and propidium
iodide using fluorescence microscopy, and the
results were confirmed using flow cytometry.

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