Fundamental techniques in virology
Author(s) : Habel, K. ; Salzman, N. P.
Editors : Habel, K.; Salzman, N. P.
Book : Fundamental techniques in virology. 1969 pp.xx+527 pp.
Abstract : This book contains 47 contributions on various aspects of virology arranged in 9 sections. Part I, on tissue culture methods, ranges from mass cell cultures in multiple tier closed systems (J. C. HOLPER: p. 3) to cell cloning in microdrops (I. MACPHERSON p. 17), with 2 papers on techniques for the synchronization of cell division. Then follow, in Part II. 4 papers on the preparation of subcellular fractions and 5 papers, in Part III, on the concentration and purification of viruses, this context being extended to include a contribution by E. R. PFEFFERKORN (p. 87) on the isolation and characterization of temperaturesensitive mutants of animal viruses. Part IV. on quantitative assay procedures for virus infectivity, contains 8 valuable papers, that by P. I. MARcus and D. H. CARVER (p. 161) on the haemadsorption-negative plaque test being a particularly clear and concise account of a method first developed to titrate rubella virus but now being applied to titrate several other " difficult " viruses.
3 short papers on assay methods in more general terms in Part V lead on to more detailed accounts in Parts VI, VII and VIII on the analysis of proteins, RNA and DNA respectively. The protein section contains a useful paper by M. S. HoawrTz and M. D. SCHARFF (p. 253) on the production of antisera against virus antigens, followed by N. J. SCHMIDT's account (p. 263) of the complement-fixation test, with special reference to the micro-modification of the method. There are 2 papers on radioactive-labelled viral proteins, and a detailed account by J. V. MAIZEL, Jr. (p. 334) of acrylamide gel electrophoresis. The final part, a single paper by A. F. HOWATSON (p. 505) on electron microscopic procedures, summarizes recent developments in this field.
It is difficult for any virologist to keep abreast of developments in his own field, let alone those in other specialties. This book will be of particular value to the virologist who wishes to apply one of the more recently developed or refined techniques, but it should also help the non-specialist to appreciate the possibilities (and some of the limitations) of these new experimental methods. J. S. Porterfield.
Record Number : 19702702616
Publisher : London. New York : Academic Press Inc.
Language of text : English
Author(s) : Habel, K. ; Salzman, N. P.
Editors : Habel, K.; Salzman, N. P.
Book : Fundamental techniques in virology. 1969 pp.xx+527 pp.
Abstract : This book contains 47 contributions on various aspects of virology arranged in 9 sections. Part I, on tissue culture methods, ranges from mass cell cultures in multiple tier closed systems (J. C. HOLPER: p. 3) to cell cloning in microdrops (I. MACPHERSON p. 17), with 2 papers on techniques for the synchronization of cell division. Then follow, in Part II. 4 papers on the preparation of subcellular fractions and 5 papers, in Part III, on the concentration and purification of viruses, this context being extended to include a contribution by E. R. PFEFFERKORN (p. 87) on the isolation and characterization of temperaturesensitive mutants of animal viruses. Part IV. on quantitative assay procedures for virus infectivity, contains 8 valuable papers, that by P. I. MARcus and D. H. CARVER (p. 161) on the haemadsorption-negative plaque test being a particularly clear and concise account of a method first developed to titrate rubella virus but now being applied to titrate several other " difficult " viruses.
3 short papers on assay methods in more general terms in Part V lead on to more detailed accounts in Parts VI, VII and VIII on the analysis of proteins, RNA and DNA respectively. The protein section contains a useful paper by M. S. HoawrTz and M. D. SCHARFF (p. 253) on the production of antisera against virus antigens, followed by N. J. SCHMIDT's account (p. 263) of the complement-fixation test, with special reference to the micro-modification of the method. There are 2 papers on radioactive-labelled viral proteins, and a detailed account by J. V. MAIZEL, Jr. (p. 334) of acrylamide gel electrophoresis. The final part, a single paper by A. F. HOWATSON (p. 505) on electron microscopic procedures, summarizes recent developments in this field.
It is difficult for any virologist to keep abreast of developments in his own field, let alone those in other specialties. This book will be of particular value to the virologist who wishes to apply one of the more recently developed or refined techniques, but it should also help the non-specialist to appreciate the possibilities (and some of the limitations) of these new experimental methods. J. S. Porterfield.
Record Number : 19702702616
Publisher : London. New York : Academic Press Inc.
Language of text : English