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JeanMarie Houghton (Editor)
Authoritative and practical, Helicobacter Species: Methods and Protocols seeks to aid scientists in further study of this crucially important research into Helicobacter research.
Contents
Preface . . . v
Contributors. . . ix
1 Introduction . . .1
2 Helicobacter pylori: An Overview . . .7
3 Perspectives on Methodology for In Vitro Culture of Helicobacter pylori . . .11
4 Successful Culture Techniques for HelicobacterSpecies: General Culture Techniques for Helicobacter pylori. . .17
5 Successful Culture Techniques for Helicobacter Species: Establishing H. pyloriCultures from Infected Rodents. . .29
6 Successful Culture Techniques for Helicobacter Species: Verification of HelicobacterIdentity Using 16S rRNA Gene Sequence Analysis... 37
7 The Helicobacter pylori cagPathogenicity Island . . .41
8 Genetic Manipulation of a Naturally Competent Bacterium,Helicobacter pylori. . .51
9 A Method for Short-Term Culture of Human Gastric Epithelial Cells to Study the Effects of Helicobacter pylori. . .61
10 Cell Culture-Based Assays to Test for Bacterial Adherence and Internalization ... 69
11 Cell Culture Assays to Evaluate Bacterial Toxicity and Virulence . . .77
12 Rodent Models of HelicobacterInfection, In fl ammation and Disease . . .89
13 Bacterial Culture and Inoculation of Mice (Simple Infection) . . .99
14 Adoptive Transfer of Splenocytes to Immunocompromised Mice. . .109
15 Isolation of Gastric Lamina Propria Leukocytes . . .113
16 Delayed-Type Hypersensitivity Determination. . .117
17 Necropsy, Blood, Tissue Collection and mRNA Isolation for Detection of Host Cytokine Gene Expression . . .119
18 Animal Models of Helicobacter-Induced Disease: Methods to Successfully Infect the Mouse . . .131
19 Verifying and Quantifying Helicobacter pyloriInfection Status of Research Mice. . .143
20 Mouse Models of Helicobacter-Induced Gastric Cancer: Use of Cocarcinogens . . .157
21 Gastric Helicobacterspp. in Animal Models: Pathogenesis and Modulation by Extragastric Coinfections. . . 175
22 Histologic Scoring of Gastritis and Gastric Cancer in Mouse Models . . .189
23 Innate Immune Responses to Helicobacter pyloriInfection: An Overview. . . . . 205
24 Methods for In Vivo and In Vitro Analysis of Innate Immune Responses to Helicobacter pyloriInfection. . . 209
25 Techniques for Following Labeled Cells In Vivo: Use of X/Y FISH, Techniques to Optimize Fluorescent Detection, and Beta-Galactosidase Detection . . . 227
26 In Vivo Measurement of Helicobacter pyloriInfection . . . 239
Index. . . 257

- Hardcover: 268 pages
- Publisher: --; 2013 edition (September 27, 2012)
- Language: English
- ISBN-10: 1627030042
- ISBN-13: 978-1627030045
- Presents methods and protocols commonly used to study Helicobacter
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice form the experts
Authoritative and practical, Helicobacter Species: Methods and Protocols seeks to aid scientists in further study of this crucially important research into Helicobacter research.
Contents
Preface . . . v
Contributors. . . ix
1 Introduction . . .1
2 Helicobacter pylori: An Overview . . .7
3 Perspectives on Methodology for In Vitro Culture of Helicobacter pylori . . .11
4 Successful Culture Techniques for HelicobacterSpecies: General Culture Techniques for Helicobacter pylori. . .17
5 Successful Culture Techniques for Helicobacter Species: Establishing H. pyloriCultures from Infected Rodents. . .29
6 Successful Culture Techniques for Helicobacter Species: Verification of HelicobacterIdentity Using 16S rRNA Gene Sequence Analysis... 37
7 The Helicobacter pylori cagPathogenicity Island . . .41
8 Genetic Manipulation of a Naturally Competent Bacterium,Helicobacter pylori. . .51
9 A Method for Short-Term Culture of Human Gastric Epithelial Cells to Study the Effects of Helicobacter pylori. . .61
10 Cell Culture-Based Assays to Test for Bacterial Adherence and Internalization ... 69
11 Cell Culture Assays to Evaluate Bacterial Toxicity and Virulence . . .77
12 Rodent Models of HelicobacterInfection, In fl ammation and Disease . . .89
13 Bacterial Culture and Inoculation of Mice (Simple Infection) . . .99
14 Adoptive Transfer of Splenocytes to Immunocompromised Mice. . .109
15 Isolation of Gastric Lamina Propria Leukocytes . . .113
16 Delayed-Type Hypersensitivity Determination. . .117
17 Necropsy, Blood, Tissue Collection and mRNA Isolation for Detection of Host Cytokine Gene Expression . . .119
18 Animal Models of Helicobacter-Induced Disease: Methods to Successfully Infect the Mouse . . .131
19 Verifying and Quantifying Helicobacter pyloriInfection Status of Research Mice. . .143
20 Mouse Models of Helicobacter-Induced Gastric Cancer: Use of Cocarcinogens . . .157
21 Gastric Helicobacterspp. in Animal Models: Pathogenesis and Modulation by Extragastric Coinfections. . . 175
22 Histologic Scoring of Gastritis and Gastric Cancer in Mouse Models . . .189
23 Innate Immune Responses to Helicobacter pyloriInfection: An Overview. . . . . 205
24 Methods for In Vivo and In Vitro Analysis of Innate Immune Responses to Helicobacter pyloriInfection. . . 209
25 Techniques for Following Labeled Cells In Vivo: Use of X/Y FISH, Techniques to Optimize Fluorescent Detection, and Beta-Galactosidase Detection . . . 227
26 In Vivo Measurement of Helicobacter pyloriInfection . . . 239
Index. . . 257