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Identi cation and Characterization of Salmonella Serotypes Isolated from Pork and Poultry from Commercial Sources
Yulie Edith Meneses
University of Nebraska at Lincoln, [email protected]
Salmonella is one of the leading causes of foodborne illness worldwide, and it is
estimated that 1.4 million infections occur annually in the U.S. alone. The Premi®Test
Salmonella (PTS), is a potential tool for rapid detection and identification of Salmonella
serovars. The objective of this project was to evaluate the use of the PTS system as a
serotyping tool to identify pork and poultry isolates obtained from vertically integrated
operations and to characterize their antibiotic resistance. In addition a risk assessment
model was proposed for future research.
Two hundred isolates were evaluated. All isolates were serotyped using the traditional
Kaufmann-White scheme and the PTS system. Among the isolates 63 different serotypes
were represented, 36 of which were included in the PTS database and 27 were not
present in it. CDC pulsed field gel electrophoresis protocol was used to characterize the
relatedness among isolates and their antibiotic resistance was determined using the
Kirby-Bauer disc diffusion test.
Serotype identification using the PTS system was reproducible independently of the
source (pork or chicken) or replication. Sixty three percent of the serotypes present in by
the PTS database were successfully indentified as Salmonella and matched traditional
serotyping. Thirty seven percent of the isolates were identified as Salmonella but did not
match results from traditional serotyping. Close relatedness among isolates was not
responsible (in most of the cases) for the mismatches between KW and PTS system from
serotypes present in the data base. Tetracycline resistance was observed mainly in pork
isolates (S. Anatum, S. Heidelberg, S.Mbandaka and S. Johannesburg). Two multidrug
resistance patterns were detected in S. Typhimurium and S. Bovis –morbificans (G-AMC
and Te-G-AM respectively).
Link:
Yulie Edith Meneses
University of Nebraska at Lincoln, [email protected]
Salmonella is one of the leading causes of foodborne illness worldwide, and it is
estimated that 1.4 million infections occur annually in the U.S. alone. The Premi®Test
Salmonella (PTS), is a potential tool for rapid detection and identification of Salmonella
serovars. The objective of this project was to evaluate the use of the PTS system as a
serotyping tool to identify pork and poultry isolates obtained from vertically integrated
operations and to characterize their antibiotic resistance. In addition a risk assessment
model was proposed for future research.
Two hundred isolates were evaluated. All isolates were serotyped using the traditional
Kaufmann-White scheme and the PTS system. Among the isolates 63 different serotypes
were represented, 36 of which were included in the PTS database and 27 were not
present in it. CDC pulsed field gel electrophoresis protocol was used to characterize the
relatedness among isolates and their antibiotic resistance was determined using the
Kirby-Bauer disc diffusion test.
Serotype identification using the PTS system was reproducible independently of the
source (pork or chicken) or replication. Sixty three percent of the serotypes present in by
the PTS database were successfully indentified as Salmonella and matched traditional
serotyping. Thirty seven percent of the isolates were identified as Salmonella but did not
match results from traditional serotyping. Close relatedness among isolates was not
responsible (in most of the cases) for the mismatches between KW and PTS system from
serotypes present in the data base. Tetracycline resistance was observed mainly in pork
isolates (S. Anatum, S. Heidelberg, S.Mbandaka and S. Johannesburg). Two multidrug
resistance patterns were detected in S. Typhimurium and S. Bovis –morbificans (G-AMC
and Te-G-AM respectively).
Link: