Isolation and identification of yeast flora from genital tract in healthy female camels

[aria.ariyayie]

Yeasts are commensal organisms found in the skin, genital and gastrointestinal tracts, and
other mucosal sites in mammalians. The purposes of this study were to identify yeast flora
and to determine the number of colony forming units (CFUs) in genital tract of healthy
female dromedary camels, establishing their connection in both mated and unmated
conditions. The samples were taken from different parts of genital tract including
vestibule, vagina, cervix, uterine body, and uterine horns of 50 camels using sterilized
cotton swabs. They were cultured onto Sabouraud glucose agar containing chloramphenicol
and incubated at 30 8C for 7–10 days. A total of 454 yeast colonies were obtained from
genital tract. Yeast isolates belonged to 8 genera: Candida (73.1%), Trichosporon (10.1%),
Geotrichum (7.5%), Kluyveromyces (3.5%), Rhodotorula (2.4%), Aureobasidium (1.4%),
Cryptococcus (1.1%) and Prototheca (0.8%). Among different Candida species, C. zeylanoides
was the most common isolated species, representing significant difference with other
Candida species (P < 0.05). The mean number of yeasts found in the vestibule (46%) was
significantly higher than the results obtained from other parts (P < 0.05). In addition, the
mean value of CFUs from unmated females (71.1%) was significantly higher than mated
females (P < 0.05). The results showed that C. zeylanoides was a common component of
healthy camel females’ genital mycoflora and the number of yeasts varied between mated
and unmated females.

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