Analysis of bacterial diversity in the intestine of grass carp

[Vetaqua]

Shaofeng Han et al.
Analysis of bacterial diversity in the intestine of grass
carp (Ctenopharyngodon idellus) based on 16S rDNA
gene sequences
Aquaculture Research, 2010, 42, 47-56


Abstract
In the current study, we assessed bacterial diversity
in the gut content of pond-reared grass carp (Ctenopharyngodon
idellus), in the associated habitat environments
(pond water and sediment) and in the
ingested food (commercial feed and the reed Phragmites
australis) by analysing 16S rDNA sequences
from clone libraries. The highest bacterial diversity
was observed in the gut content and was determined
by the total number of operational taxonomic units,
Shannon diversity index (H), Shannon equitability
index (EH), Coverage (Cgood) and rarefaction curves
calculated from the 16S rDNA gene libraries. Our
data indicated that allochthonous gut microbes of
grass carpwere distinctively di¡erent fromthe corresponding
environmental microbes. The pairwise similarity
coe⁄cient (Cs) for microbe communities
between gut content and ingested food was higher
than for those between the gut content and habitats,
indicating that the allochthonous microbiota identi-
¢ed in the intestines of grass carp were phylogenetically
closer to those in the ingested food than to those
in the habitat. Based on our study and previous
research, we suggest that the digesta of grass carp
harbours a microbiota phylogenetic core of Proteobacteria
and Firmicutes and this observation
deserves further investigations with respect to a potential
pool of probiotics to grass carp.

Keywords: grass carp, Ctenopharyngodon idellus,
intestinal bacterial diversity,16S rDNA

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